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KMID : 1140120120170030251
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Cancer Prevention Research
2012 Volume.17 No. 3 p.251 ~ p.256
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Protective Effects of Ethylacetate Fraction from Taraxacum coreanum against Peroxynitrite-induced Oxidative Damage under Cellular System
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Choi Ji-Myung
Choi Mi-Jin
Lee Sul-Lim
Yamabe Noriko
Lee Sang-Hyun
Cho Eun-Ju
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Abstract
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The EtOAc fraction from Taraxacum coreanum showed the strongest radical scavenging activity. The protective activity of active EtOAc fraction against the oxidative stress was investigated under cellular system using LLC-PK1 renal epithelial cell. The LLC-PK1 cells showed loss of the cell viability and elevation for lipid peroxidation by the treatment with generator of peroxynitrite (ONOO－), 3-morpholinosydnonimine, and its precursors, nitric oxide (NO) and superoxide anion (O2 －), produced by sodium nitroprusside and pyrogallol, respectively. However, the treatment of the EtOAc fraction significantly, dose-dependently, recovered cell viability and inhibited lipid peroxidation against both ONOO－ itself, and its precursors of NO and O2 －. At 100μg/ml of the EtOAC fraction, the cell viability was significantly increased to 93.4% from 61.0%, and lipid peroxidation was inhibited to 0.104 nmol/mg protein from 0.494 nmol/mg protein. The present study suggests that T. coreanum would have the protective potential from ONOO－-induced oxidative stress.
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KEYWORD
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Taraxacum coreanum, LLC-PK1, Peroxynitrite, Superoxide anion, Nitric oxide
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